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Deering High School students visit the Marine Science Center at the University of New England.

Engaging students in STEM can be challenging, especially with the limited resources of most classrooms.  That’s why Karen Shibles, a Deering High School science teacher, took advantage of a unique opportunity offered by iXplore and enlisted her students in the “Maine Barcode of Life” (MBOL) project. This project focuses on biodiversity and genetics and connects students to the living world around them.

During the first phase of the project, students learned about marine organisms by visiting the University of New England’s (UNE) Marine Science Center.  Dr. Markus Frederich and Dr. Deborah Landry gave the visitors a tour of the marine biology labs with expansive touch tanks. At first, the students were very apprehensive (even scared) about getting too close to live marine organisms, but with the help of UNE students, the high schoolers slowly began exploring the saltwater tanks and holding starfish, crabs, and urchins.

A skate startles a group of students.

Determining the exact species of fish, crab or sea star can be very difficult, even with a field guide, unless you’re experienced.  This was especially true for the Deering students since most of them had never been to the ocean. During the second phase of the MBOL project, students learned how to use DNA barcoding, a powerful research method which can identify and classify any organism. So how does DNA barcoding work? First, students extract DNA from the organism through a series of steps using different chemicals and equipment. Second, students use biotechnology to amplify one universal gene and later obtain the DNA sequence or code. Third, students import the DNA code into the BOLDsystems website, which compares the code to all the other codes stored in the genetic database. Within seconds, BOLDsystems reports the species name and how it relates to all other species. In the end, Deering High School students learned about marine biodiversity, advanced their lab and analytical skills, and published 8 new species records.  Thank you Deering High School for contributing to the Maine Barcode of Life!

“What can boring fish eggs teach us?” a 4th grader asked while looking at the motionless eggs at the bottom of the tank. “I thought you were bringing real fish into the classroom”.

Little embryos growing, changing

Little fish eyes keep watch!

“Just you wait!” I said hoping he would reconsider after watching the eggs transform over the next three months.

My goal, as STEM curriculum developer, was to bring into focus how organisms grow and develop.  Studying Atlantic salmon, from eggs to fish, would provide a model for the study of life cycles, traits, behavior, natural selection, and the influence of the environment. The Yarmouth Elementary Salmon Hatchery offered a unique, hands-on experience for students and educators, who might otherwise never get up-close to such fascinating creatures that once lived abundantly in the nearby Royal River. Students also applied math skills when predicting “Hatch-Out” day and estimating the relative amounts of ocean, glacier, ground and fresh water on Earth.

After the students released the young salmon fry many miles upriver from Yarmouth, they asked new questions….Will they find enough food? Will they survive? Will they return to the Royal? Although I knew we couldn’t answer most of these questions, their experience clearly stimulated thinking beyond the classroom.

In the end, the 4th graders made keen observations, asked questions, tested predictions, and shared conclusions in conversations and writing about the growth and development of salmon. Their first hand experience observing, caring for, and learning about an endangered species, will likely make a lasting impression and potentially create a new generation of environmental stewards.

– by Deborah Landry, Ph.D.

Video Playlist

Project Sponsors: Atlantic Salmon Federation, Saco Salmon Club, Yarmouth Education Foundation, Yarmouth School District and iXplore STEM



What a surprise to find a post about the Maine Barcode of Life (MBOL) project coming all the way from Canada!  Dirk Steinke, a DNA barcoding expert from the Biodiversity Institute of Ontario at the University of Guelph writes a very complementary post about our work. iXplore launched MBOL in 2013 and recently, with a generous donation from the Maine Community Foundation Rine-Thompson fund, we will broaden student participation in Greater Portland, Maine. The grant provides the necessary funds to complete the “DNA Barcoding Foot Locker” containing equipment and supplies. The foot locker will travel to high schools to support student research during the academic year. Thanks for the shout out Dirk… and you’re right, we do like to have fun!


IXplore STEM was awarded a generous grant from the Rines-Thomson Fund of the Maine Community Foundation to broaden student participation in science, technology, engineering and math (STEM). This grant provides high school students (in the Greater Portland area) the opportunity to engage in modern scientific research using DNA barcoding and contribute to the Maine Barcode of Life project (MBOL 2015).  Funds will go towards the purchase of equipment and supplies to create a “DNA Barcoding Footlocker”, which can travel from school to school for classroom research. IXplore STEM launched the Maine Barcode of Life project in 2013, which is a joint effort by Maine high schools and colleges to assess the biodiversity, distribution and abundance of Maine’s marine and land-based organisms and to build a genetic library of Maine species – past, present, and future.


Students use biotechnology in their research projects.

“Life science students and teachers will benefit from this project – using modern biotechnology and equipment that would otherwise not be available to them,” stated Karen Shibles, a Deering High School science teacher who completed training during an iXplore summer workshop. “I am convinced that such experiences will build the kind of 21st century STEM skills, knowledge and confidence that our students need.”

“Students investigate topics such as the effects of global warming on biodiversity, the presence of invasive species, and food fraud in markets and restaurants,” said Deborah Landry, Executive Director of iXplore STEM. “Students publish their results in and build the genetic library of existing Maine species that can be used by scientists around the globe.”

The University of New England’s Marine Science Center will collaborate on the project and host classroom visits to collect specimens for student research.

Collecting Specimens Saco Bay

Students and educators collect specimens for research while visiting the UNE Marine Science Center.

“This effective teaching tool not only offers an avenue for authentic classroom research, but it also prepares students for college level science,” commented Markus Frederich, a UNE professor of marine biology.

IXplore STEM partners with institutes of higher learning, businesses, and other nonprofits to develop programs that encourage students to pursue STEM degrees and promote STEM awareness in the community. To learn more about student and educator programs, visit

The Maine Community Foundation is located in both Ellsworth and Portland and works with donors and other partners to improve the quality of life for all Maine people. Visit to learn more about the foundation.